Abstract
Eight modifications of hemagglutination technique were applied to the sensitization of sheep erythrocytes with Au antigen; these included tannic acid (TA), bis-diazotized benzidine (BDB), tolylene-2, 4-diisocyanate (TDIC), difluorodinitrobenzene, tetraazotized O-dianisidine (TOD), cyanulic chloride5), glutaraldehyde and chromic chloride (CrCl3) method.The sensitized cells could be obtained only using TA, BDB, TDIC or TOD in the glutaraldehyde-treated cells and CrCl3 in the native cells, if the concentration of Au antigen in the reaction solution was limited to about 25γ/ml. Among these reagents the best result was obtained with BDB.The coupling of Au antigen to erythrocytes with these reagents seems not significantly to inactivate the antigenic groups of Au antigen.The cells coated with the aid of BDB, TDIC or TA could be used again without any appreciable loss of hemagglutination titer for 4 months when preserved at 4°C.
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