Atypical Leuconostoc-like Weissella Strains isolated from Meat, Sharing Low Phenotypic Relatedness with the so far Recognized Arginine-negative Weissella spp. as Revealed by SDS-PAGE of Whole Cell Proteins

Abstract

Summary The levels of phenotypic relatedness among 34 atypical, arginine-negative Weissella strains isolated from naturally fermented Greek dry salami and the species Weissella paramesenteroides (type strain), Weissella hellenica (five strains including type strain), Weissella viridescens (four strains and a reference strain) and Leuconostoc mesenteroides subsp. mesenteroides (one strain) were determined by numerical analysis of whole cell protein profiles. W. paramesenteroides was phenotypically more closely related to Lc. mesenteroides rather than to W. hellenica and W. viridescens. Clearly separated from these four species, atypical Weissella strains formed three distinct clusters, which were also of low phenotypic similarity to each other. Two of the clusters were tight and comprised strains capable of producing solely D-lactate. The third cluster was very diverse as consisted of four subgroups and an “intruder” strain. Most strains of this cluster formed D(L)-lactate. Based on the SDS-PAGE findings, evidence was provided that atypical sausage isolates may represent new, unidentified Weissella taxons of meat origin.