Attenuation of δ opioid receptor-mediated signaling by kainic acid in neural cells: involvement of protein kinase C and intracellular Ca 2+

Abstract

The potential modulation of opioid receptor signaling by kainic acid (KA) has been investigated in neuroblastoma×glioma NG 108-15 hybrid cells and neuroblastoma SK-N-SH cells. Acute incubation of KA significantly attenuated δ opioid receptor (DOR) signaling induced by the DOR agonist [ d-Pen 2, d-Pen 5]-enkephalin (DPDPE), as measured by activation of G proteins and inhibition of cAMP accumulation. The attenuation by KA was time- and dose-dependent and could be blocked by antagonists of kainate/AMPA receptors, suggesting possible mediation through kainate/AMPA receptors. KA attenuation of DPDPE-stimulated G protein activation was reversed by inhibitors of protein kinase C or by removal of both extracellular Ca 2+ and intracellular Ca 2+. In contrast, NMDA attenuation of DPDPE-stimulated G protein activation was independent of intracellular Ca 2+, indicating that different mechanism(s) may underlie the modulation effect of KA and NMDA. This notion was further supported by the results that KA did not alter nociceptin/orphanin FQ-stimulated G protein activation in NG 108-15 cells but NMDA did. In addition, pretreatment of NG 108-15 cells with antagonists of kainate/AMPA receptors blocked the acute desensitization of DOR signaling. These data provide evidence that KA may be involved in the modulation of opioid receptor signal transduction.