Abstract
Optical properties, such as the attenuation coefficients of multi-layer tissue samples, could be used as a biomarker for diagnosis and disease progression in clinical practice. In this paper, we present a method to estimate the attenuation coefficients in a multi-layer sample by fitting a single scattering model for the OCT signal to the recorded OCT signal. In addition, we employ numerical simulations to obtain the theoretically achievable precision and accuracy of the estimated parameters under various experimental conditions. Finally, the method is applied to two sets of measurements obtained from a multi-layer phantom by two experimental OCT systems: one with a large and one with a small Rayleigh length. Numerical and experimental results show an accurate estimation of the attenuation coefficients when using multiple B-scans.
Highlights
Optical coherence tomography (OCT) is an interferometric imaging technique that can generate high-resolution three-dimensional images of biological tissues
The coefficient of variations (CoV) of the estimated attenuation coefficients of four layers and the corresponding estimation bias are shown in Fig. 2(c-f) as a function of focus location for the two selected Rayleigh lengths
For zR = 40 μm, the bias of the estimated attenuation coefficients remains below 10% when the focus location is less than 0.5 mm inside the sample
Summary
Optical coherence tomography (OCT) is an interferometric imaging technique that can generate high-resolution three-dimensional images of biological tissues. Stefan et al [16] introduced a method to estimate the attenuation coefficient using two B-scans with different focus locations to first estimate the location of focus and subsequently estimate the attenuation coefficient from a single scattering model of the OCT light after compensating for the effect of beam shape. This method is dependent on having identical lateral beam locations of the sample to unambiguously determine the effect of the beam. There is a need, e.g. in ophthalmology, for a method to reliably estimate the attenuation coefficient properties in multi-layer samples
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