Abstract
Attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) has recently been proven to be a powerful tool for bioanalysis. It enables in situ and in real-time observation of dynamic processes occurring on specific interface, revealing rich structural and functional information of biomolecules at sub monolayer level. The aim of this general review was to give an overview of the cutting edge applications of ATR-SEIRAS. We start with description of the basic configuration of the standard ATR-SEIRAS platform. The enhanced mechanisms and methods to fabricate enhanced substrates are then presented. We discuss the recent developments, challenges and applications of ATR-SEIRAS in bioanalysis, mainly focusing on DNA analysis, protein behavior and cell properties. Finally, further development of the ATR-SEIRAS technique with enhanced sensitivity, improved time and spatial resolutions will be prospected.
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