Abstract
Previously, Shigella carrier 15D was shown to deliver a mammalian DNA expression plasmid to cultured cells with subsequent production of the plasmid-encoded foreign protein. In this study, we report in vivo delivery of a DNA expression plasmid to mucosal tissue results in the stimulation of immune responses against the plasmid-encoded foreign antigen. Splenocytes from mice receiving two intranasal inoculations of 15D carrying pCMVβ showed proliferative responses to the plasmid-encoded Escherichia coli β-galactosidase. In addition, antibody specific for β-galactosidase was detected in pooled sera collected from 15D(pCMVβ) infected mice.
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