Abstract
Summary And ConclusionAttempts to grow Leishmania donovani were made in cultures of chicken, hamster and human tissues. It was found that the parasites were taken up by the phagocytes within 24 hours. In the hanging drop cultures parasites were all degenerated by the 15th day. In the slowly growing cultures in flasks prepared according to Fischer and Parker's method they were all degenerated by the 43rd day. Multiplication of the parasites has not been observed.It is concluded that the parasites may survive for various lengths of time but cannot grow in the tissue cultures according to the methods described, and that the length of survival is in inverse ratio with the rapidity of the growth of the tissue cultures.
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