Attachment, uncoating and blockage of cell protein synthesis by Sabin derivatives of poliovirus in HeLa and Vero cells.

Abstract

Virulent Mah type 1 poliovirus and the attenuated Sab types 1, 2 and 3 poliovirus do not efficiently produce plaques or grow as efficiently with Vero cells as with HeLa cells. All viruses attach poorly to simian cells on monolayer and, except for Mah and Sab1, eclipse poorly. Additionally, all three attenuated viruses block initiation of Vero cell protein synthesis less effectively than HeLa cell protein synthesis. The lower rates of attachment and eclipse of these viruses with Vero cells and their limited capacities to block cellular protein synthesis and thereby provide ribosomes for translation of viral RNA probably contribute to their more restricted growth in Vero cells.