Attachment of indium-111 labelled endothelial cells to pretreated polytetrafluoroethylene vascular grafts

Abstract

This study investigated the effect of different surface coatings on endothelial cell attachment to polytetrafluoroethylene (PTFE) vascular grafts. Small segments of PTFE vascular grafts were precoated with one of a number of substances: gelatin, poly-l-lysin, fibronectin, or collagen type I, III or IV. Indium-111 labelled endothelial cells were then seeded on to the grafts and left for either 10, 30, 60 or 120 min. The unattached cells were removed and the degree of cell attachment was calculated. All coatings were significantly better at enhancing endothelial cell attachment at all times compared with controls, and fibronectin was significantly better as a coating material than any other material used. By pretreating with a substance such as fibronectin, the number of endothelial cells attaching to PTFE vascular grafts can be greatly increased, thereby enhancing the cell seeding process.