Abstract
Mechanically isolated plant cells provide a convenient single-cell system for investigating bacterial attachment during the early stages of transformation by Agrobacterium tumefaciens. Cells isolated from cladodes of Asparagus officinalis were aggregated by both virulent and non-virulent strains of Agrobacterium, indicating that the bacterium can attach to the walls of cells from this Monocotyledon. The effects of temperature, media, cell concentration and viability were investigated in relation to the attachment process.
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