Abstract
Atrial natriuretic peptide (ANP) is a cardiac hormoneinvolved in the regulation of fluid balance and bloodpressure homeostasis. Using conventional reversetranscription and polymerase chain reaction andamplification, preproANP transcript from Ranaridibunda heart ventricle was cloned andcharacterized. Sequence analysis of the completecDNA revealed an open reading frame for a 146-residue peptide, carrying a 17-residue ring at the Cterminus,characteristic of the natriuretic peptidefamily. Perfusion of Rana ridibunda myocardiumunder diverse stimuli such as mechanical,hyperosmotic or oxidative stress induced a rapidincrease in the transcripts, which is accompanied bya significant increase in phosphorylated p38-MAPKlevels. Furthermore, 1 µM SB203580, the selectivep38-MAPK inhibitor, was found to abolish this increasesuggesting the involvement of the p38-MAPKsignalling pathway in the regulation of ANP mRNAexpression.
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