Abstract
BackgroundMany studies are in the literature on the ANP and oxytocin-presence in the brain, but very few studies with controversial results are reported on the presence of these peptides in the cerebellum. This immunohistochemical study investigates on the ANP and oxytocin-presence in the cerebellum of the adult rat and mouse rodents.ResultsThis study, firstly, evidences the ANP- immunopositivity in cerebellar cortex of both rat and mouse rodents. In rat the molecular layer presents some few immunopositive fibers, but no neuron resulted immunopositive; the granular and Purkinje cells are immunopositive. In mouse the cerebellar cortex ANP-immunopositivity is present in all layers. The oxytocin-presence in the rat the afferent fibers are immunopositive are in the granular layer; in mouse the OT-immunopositivity is in the molecular layer only.ConclusionsThis study, firstly, shows that ANP and OT are present in the cerebellar cortex both in rat and mouse rodents. In the mouse cerebellar cortex ANP-presence is more diffuse and OT- localization differences in the two species.
Highlights
Many studies are in the literature on the Atrial natriuretic peptide (ANP) and oxytocin-presence in the brain, but very few studies with controversial results are reported on the presence of these peptides in the cerebellum
Since the studies reported controversial results regarding the ANP presence in the cerebellum of the different species, this our study aims to investigate, by immunohistochemical method, the presence and localization of ANP in adult rat and mouse cerebellum, since our opinion is that ANP may be involved in the modulation of some cerebellum activities, i.e.by paracrine/autocrine mechanisms
ANP-immunostained sections showed that the rat cerebellum presents, into the lamellae of the white substance, numerous compacted fascicles of fibers that were strongly ANP-immunopositive; these fibers penetrated and subdivided in the granular layer, many fibers crossed the granular layer and their extremities surrounded the cell body of the Purkinje cells
Summary
Many studies are in the literature on the ANP and oxytocin-presence in the brain, but very few studies with controversial results are reported on the presence of these peptides in the cerebellum. This immunohistochemical study investigates on the ANP and oxytocin-presence in the cerebellum of the adult rat and mouse rodents. In rat cerebellum no ANP-positive cell was found [1]; regarding the ANPbinding sites the results are controversial, Gibson et al [2], by autoradiography, reported the presence of the ANP-binding sites in low density, while Manthy [3] reported the absence of ANP-binding sites.
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