Abstract
This study used Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy to provided detail information of the organic functional group of secondary metabolites, produced by ferns. The study showed ATR-FTIR spectra of nine fern species: Asplenium scolopendrium, Asplenium trichomanes, Asplenium trichomanes-ramosum, Athyrium filix-femina, Dryopteris affinis, Dryopteris filix-mas, Polypodium vulgare, Polystichum lobatum and Phegopteris connectilis. After comparison of each plant FTIR region divided in three areas: 2800-3000 cm-1 (aliphatic region), 1600-1800 cm-1 (distinct oxygenated groups) and 700-900 cm-1 (aromatic bands). ATR-FTIR Spectroscopy combined with multivariate data analysis have been applied for the discrimination of 9 fern species.
Highlights
This study used Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy to provided detail information of the organic functional group of secondary metabolites, produced by ferns
The FTIR spectra of nine species of ferns are shown in 1240 cm-1, while for Polypodium vulgare (7), Polystichum figures 1 and preliminary assignments are present in table
Fig. 2. 2-D scores obtained from Principal Component Analysis (PCA) of FTIR spectra of ferns 1-8 for the first two principal components (PCs) based on the FTIR regions: 700-900 cm-1 1600-1750 cm-1 and 2800-300 cm-1
Summary
Leaves were collected from ferns that grow in the Valsan Valley (August 2018). The nine species of fern are: Asplenium scolopendrium L. (Woodsiaceae) (4), Dryopteris affinis (Dryopteridaceae) (5), Dryopteris filix-mas (Dr yopteridaceae) (6), Polypodium vulgare L. (Polypodiaceae) (7), Polystichum aculeatum (L.) Roth (Dryopteridaceae) (8) and Phegopteris connectilis (Minchx) Watt (Thelypteridaceae) (9) [3]. The leaves were dried at room temperature. The spores were stored both at room temperature (1’, 2’ 3’, 9’) and refrigerated at 4°C (1’’, 2’’, 3’’) [4]
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