Abstract
Atractylodin is one of the major constituents of the rhizome of Atractylodes lancea, which is widely used in Korean traditional medicine as a remedy for the treatment of gastritis and gastric ulcers. Despite of a major constituent of widely used botanical to treat inflammatory responses little is known about anti-inflammatory effect of atractylodin in the human mast cell (HMC-1). Hence, we evaluated the effect of atractylodin on the release of IL-6, the involvement of nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) and mitogen-activated protein kinases (MAPKs) in phorbol-12-myristate-13-acetate and A23187-induced HMC-1. In addition, Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), phospholipase C (PLC) gamma 1, and AKT phosphorylation relevant to NPM-ALK signal pathway were assessed. IL-6 levels in the HMC-1 stimulated by phorbol-12-myristate-13-acetate and A23187 were apparently decreased by the treatment of atractylodin. Concurrently, atractylodin not only inhibited the phosphorylation of NPM-ALK, but also suppressed the phosphorylation of JAK2, STAT3, PLC gamma 1, and AKT. Furthermore, the activated mitogen-activated protein kinases (MAPKs) by phorbol-12-myristate-13-acetate and A23187 were inhibited by atractylodin. These results suggested that atractylodin might have a potential regulatory effect on inflammatory mediator expression through blockade of both the phosphorylation of MAPKs and the NPM-ALK signaling pathway.
Highlights
Atractylodes lancea (Thunb.) DC. (Asteraceae) has been widely used in various Korean traditional medicine prescriptions for the treatment of gastric disorders and gastric ulcers in eastern Asia [1]
signal transducer and activator of transcription 3 (STAT3) were detected by phospho- and total nucleophosmin-anaplastic lymphoma kinase (NPM-Anaplastic lymphoma kinase (ALK)), AKT, PLCγ1 and STAT3 were detected by immunoblot analysis (C); immunoblot analysis (C);the
PLCγ is a downstream target of NPM-ALK, which contributes to its mitogenic to its mitogenic activity
Summary
Atractylodes lancea (Thunb.) DC. (Asteraceae) has been widely used in various Korean traditional medicine prescriptions for the treatment of gastric disorders and gastric ulcers in eastern Asia [1]. JAK/STAT, and PLCγ activation, and phosphorylation of ALK is involved in FcεRI transcription via to its downstream pathways [18]. NPM-ALK, turn, induces interleukins activation of recruitment to its downstream pathways [18]. ALK fusion genes are fusion associated with anaplastic therapeutic to modulate interleukins Genes are both associated with large-cell lymphoma and lymphoma inflammatory tumors [21]. Atractylodin was evaluated for its inhibitory effect on IL-6 the NPM-ALK pathway. Atractylodin was evaluated for its inhibitory effect on production and activation of the NPM-ALK and MAPK pathways in PMA/A23187-induced HMC-1. IL-6 production and activation of the NPM-ALK and MAPK pathways in PMA/A23187-induced cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
