Abstract
Atractylenolide II (AT-II) exhibits several biological and pharmacological functions, especially anti-cancer activity as the major sesquiterpene lactones isolated from Atractylodes macrocephala (also named Baizhu in Chinese). However, the effects and mechanisms of AT-II on human gastric cancer remain unclear. Cell Counting Kit-8 (CCK-8) assay, morphological changes, flow cytometry, wound healing assay and Western blot analysis were used to investigate the effects of AT-II on cell proliferation, apoptosis and motility of human gastric carcinoma cell lines HGC-27 and AGS. Our results indicated that AT-II could significantly inhibit cell proliferation, motility and induce apoptosis in a dose and time-dependent manner. Western blot analysis showed that the expression level of Bax was upregulated and the expression levels of B-cell lymphoma-2 (Bcl-2), phosphorylated-protein kinase B (p-Akt) and phosphorylated-ERK (p-ERK) were downregulated compared to control group. In conclusion, the findings suggested that AT-II exerted significant anti-tumor effects on gastric carcinoma cells by modulating Akt/ERK signaling pathway, which might shed light on therapy of gastric carcinoma.
Highlights
Gastric cancer is the fifth most common cancer and the second leading cause of cancer-related deaths in the world [1,2]
The purpose of our study is to investigate the effects of Atractylenolide II (AT-II) on cell proliferation, motility and apoptosis of gastric carcinoma cells and its possible molecular mechanisms, which would provide valid data for the application of AT-II to treat gastric carcinoma in the future
The purpose of our study is to investigate the effects of AT-II on cell proliferation, motility and apoptosis of gastric carcinoma cells and its possible molecular mechanisms, which would provide apoptosis of22,gastric carcinoma cells and its possible molecular mechanisms, which would provide
Summary
Gastric cancer is the fifth most common cancer and the second leading cause of cancer-related deaths in the world [1,2]. Atractylodes macrocephala belongs to the composite family has been an important traditional herbal medicine in Asia, which is widely used to treat dyspepsia, diarrhea, stomach diseases, diabetes and anti-abortion [9,10,11,12]. It is popularly used as heath cultivating food. The purpose of our study is to investigate the effects of AT-II on cell proliferation, motility and apoptosis of gastric carcinoma cells and its possible molecular mechanisms, which would provide valid data for the application of AT-II to treat gastric carcinoma in the future. Valid data the valid data for the application of AT-II to treat gastric carcinoma in the future
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