Year-end Sale % OFF 
Abstract
E3 ubiquitin ligase plays a vital role in the ubiquitin-mediated heat-related protein degradation pathway. Herein, we report that the expression of AtPPRT1, a C3HC4 zinc-finger ubiquitin E3 ligase gene, was induced by heat stress, and the β-glucuronidase (GUS) gene driven by the AtPPRT1 promoter has shown increased activity after basal and acquired thermotolerance. To further explore the function of AtPPRT1 in heat stress response (HSR), we used the atpprt1 mutant and AtPPRT1-overexpressing lines (OE2 and OE10) to expose in heat shock. In this study, the atpprt1 mutant had a lower germination and survival rate than those of Col-0 when suffered from the heat stress, whereas OEs enhanced basal and acquired thermotolerance in Arabidopsis seedlings. When compared to Col-0 and OEs, loss-of-function in AtPPRT1 resulted in lower chlorophyll retention and higher content of reactive oxygen species (ROS) after heat treatment. Moreover, the transcript levels of AtPPRT1 and several heat-related genes (AtZAT12, AtHSP21 and AtHSFA7a) were upregulated to greater extents in OEs and lower extents in atpprt1 compared to Col-0 after heat treated. Hence, we suggest that AtPPRT1 may act as a positive role in regulating the high temperature by mediating the degradation of unknown target proteins.
Highlights
In field production, high temperatures can cause devastating damage to crop yields [1,2]
To investigate the expression pattern of AtPPRT1 in heat stress response (HSR), 7-day-old Col-0 seedlings were exposed to heat stress
GUS histochemical staining was performed on 7-day-old AtProPPRT1::GUS transgenic plants after basal or acquired thermotolerance
Summary
High temperatures can cause devastating damage to crop yields [1,2]. In the process of plant growth, high temperatures will cause endosperm damage and cell death, thereby reducing the germination rate of seeds [4]. During the seedlings of the plant, heat stress will cause cells to lose water and reduce volume, which makes the plants dwarf, enter reproductive growth in advance and greatly reduce yield [5]. Temperature affects the activity of various enzymes, which in turn regulates the cell’s metabolic activity. These are the behaviors of plant cells in response to heat stress [7,8]. Plants have evolved different signal pathways in response to heat stress environments
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
