Abstract
The ATP2A2 gene encodes the SERCA protein required for active calcium reuptake to the sarcoplasmic reticulum in cardiac and slow-twitch skeletal muscle. The ATP2A2 rs3026468 variant has been associated with voluntary strength phenotypes in humans but requires further validation. Here we investigated a homogenous cohort of 80 young, healthy, active Caucasian males who were assessed for maximal isometric strength, voluntary activation, stimulated contractile properties, and muscle potentiation in the quadriceps. A dynamometer was used to record knee extensions, and electrical stimulation was applied to the thigh to elicit a twitch response. DNA was isolated from cheek swabs, and the rs3026468 genotypes were assessed by TaqMan primer quantitative PCR. The results show no association between ATP2A2 rs3026468 variants and muscle strength measures. We conclude there is no effect of the rs3026468 variant in our cohort and that functional influences do not likely contribute to contractile property differences in young healthy men.
Highlights
Few investigations have performed comprehensive physiological analysis to validate single nucleotide polymorphisms (SNPs) associated with skeletal muscle strength in humans
If the ATPase sarcoplasmic/endoplasmic reticulum Ca2ϩ transporting 2 (ATP2A2) rs3026468 variant does influence voluntary strength during isometric or dynamic contractions via Ca2ϩ handling during the contraction-relaxation cycle (5), we hypothesize there would likely be functional differences detected in electrically stimulated contractile properties and muscle potentiation
We investigated the involvement of the rs3026468 variant in voluntary strength, voluntary activation, stimulated contractile properties, and muscle potentiation in our cohort
Summary
Few investigations have performed comprehensive physiological analysis to validate single nucleotide polymorphisms (SNPs) associated with skeletal muscle strength in humans. If the ATP2A2 rs3026468 variant does influence voluntary strength during isometric or dynamic contractions via Ca2ϩ handling during the contraction-relaxation cycle (5), we hypothesize there would likely be functional differences detected in electrically stimulated contractile properties and muscle potentiation. We investigated the involvement of the rs3026468 variant in voluntary strength, voluntary activation, stimulated contractile properties, and muscle potentiation in our cohort.
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