Abstract
Membrane vesicles formed from spheroplasts of E. coli lysed in the presence of ADP and P i produced ATP when an artificial proton gradient (acid outside) was formed across the membrane. ATP synthesis required Mg 2+ and ADP, was inhibited by dicyclohyxylcarbodiimide and carbonyl cyanide p-trifluoromethoxyphenylhydrazone, and stimulated by valinomycin in the presence of KCl. Synthesis was absent in a mutant lacking the Mg 2+-ATPase. The optimum external pH was 2.5 when the internal pH was 8.2. Oxidative phosphorylation driven by D-lactate or succinate was also observed.
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