Abstract
Locus coeruleus (LC) is the significant nucleus for consciousness and it is sensitive to metabolic inhibition. We investigated the effects of a metabolic inhibitor sodium cyanide (NaCN) on the rat dissociated LC neurons using nystatin-perforated patch recordings. Under voltage-clamp ( V H=−40 mV), application of NaCN evoked outward currents composed of ATP-sensitive and Ca 2+-dependent K + channel currents ( I K ATP and I K Ca 2+ ). Onset of I K ATP was faster than that of I K Ca 2+ . Prolonged application of NaCN brought I K ATP rundown but not I K Ca 2+ rundown. Okadaic acid prevented I K ATP rundown, indicating that K ATP channels are deactivated by dephosphorylation with protein phosphatase.
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