Abstract
The purpose of this study was to determine the feasibility of measuring ATP released from sympathetic nerve endings in arterioles. Using F344 rats (2‐3 months old), the gastrocnemius 1A arterioles from the red gastrocnemius muscle were dissected and cannulated with glass pipettes in a tissue bath. ATP probes were connected to a potentiometer, polarized, zeroed, and a normal ATP curve generated (6.25µM to 50 µM ATP). The ATP and null probes were then positioned in the tissue bath such that the tips of the probes were submerged in the physiological saline solution and the tips were touching the arteriole in similar anatomical locations. The arterioles were then field stimulated at 60Hz for 2000 impulses at 9mA using a constant current stimulator. The normal curve produced 7.19mA at 50 µM ATP, 6.09mV at 25 µM ATP, 3.83mA at 12.5 µM. Following linear curve fitting, the slope of the line was 7.15x10−2 mA/uM ATP and an intercept of 3.22mA. Field stimulation at 60Hz produced 3.44mA that corresponded to 3.01 µM of ATP released. These data suggest that it is possible to measure ATP released from the sympathetic nerve endings from arterioles using potentiometry.
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