Abstract
The effect of sorbitol treatment on the cell structure of Candida boidinii No. 2201 was studied by transmission electron microscopy (TEM). The high ATP-producing activity of the cells was proved to be due to the plasmolysis without destruction of essential organelles for the ATP-producing system. To prepare active cells constantly, the essential elements of the yeast extract in the culture medium were identified: biotin was an essential growth factor; Fe2+ and thiamine stimulated more or less the growth; and Zn2+ strongly stimulated the growth. Limitation of Zn2+ in the culture medium (0.5mg/l of ZnCl2) improved the ATP yield from AMP, the conversion rate being 92%, possibly due to repression of AMP deaminase, for which Zn2+ was a cofactor and which affected the ATP-producing activity. A Zn2+ -supplemented culture provided fully-active cells as to ATP production. TEM revealed that Zn2+ was a necessary factor for the formation of peroxisomes in which alcohol oxidase and catalase were localized.
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