ATP-dependent Cl − uptake by plasma membrane vesicles from the rat brain

Abstract

Uptake of Cl − by plasma membrane vesicles from the rat brain was stimulated by ATP at 37°C, but not by β, γ -methylene ATP or at 0°C. The addition of Triton X-100 or sucrose to the incubation medium diminished the ATP-stimulated Cl − uptake, suggesting that Cl − was transported across the membranes into the intravesicular space. This ATP-stimulated Cl − uptake was not affected by 1 mM ouabain, 1 μM oligomycin, 0.1 mMγ -aminobutyric acid or 0.1 mM picrotoxin. Thus, non-mitochondrial ATP-driven Cl − transport through a system other than Na, K-ATPase or Cl − channels occurs in neuronal plasma membrane vesicles.