Abstract
The effects of nitrate and ammonium additions on the ATP contents of eight axenically cultured species of nitrogen deficient algae were studied to determine the reliability of short term (4 h) ATP responses in identifying nitrogen deficiency in algal populations. Addition of either nitrate or ammonium to nitrogen deficient cultures significantly increased cellular ATP content (20–30%) in all bioassays. The magnitude of the response was not affected by the source of nitrogen used. Increases in ATP content were also observed following the addition of ammonium to natural assemblages of phytoplankton. The magnitude of the response declined when samples were concentrated onto membrane filters prior to the extraction of ATP.
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