Atomic absorption determination of copper and zinc in pharmaceutical preparations

Abstract

The effect of Triton X-100 surface-active substance on the value of the analytical signal during the atomic absorption determination of copper and zinc was studied. It was shown that the maximum value of the analytical signal in the determination of analytes is achieved when Triton X-100 is used at a mass fraction of 5%. The sensitivity of the atomic absorption determination of copper increases by 1.74 times and that of zinc by 1.58 times. Sample preparation of pharmaceutical substances was carried out using ultrasound treatment. The most complete extraction of metals was achieved when the samples were treated with ultrasound for 25 minutes, and homogeneous solutions were obtained. The use of acetylacetonates of metals as standard samples of the composition increased the accuracy of copper and zinc determination due to the similarity of the calibration and analyzed solutions. The correctness of the result of the atomic absorption analysis was checked by the "injected-found out" method and by varying the weight of the sample. It was established that the systematic error is not significant. The limits of detection of copper (Cmin=0.002 g/ml, Ctheor=0.004 g/ml) and zinc (Cmin=0.003 g/ml, Ctheor=0.004 g/ml) were determined by the atomic absorption method, they are lower than those given in literature.