Abstract
In this study, we present some of the laboratory measurements of the viability of bioaerosols together with different environmental dust to understand the interaction among those components. Model airborne bacteria, DH5α Escherichia coli, was used to assess the dust affecting a viability reduction rate of the DH5α bacteria in a Teflon reaction chamber. The viability reduction rate of the DH5α model airborne bacteria was measured with a culture method. The DH5 α bacteria were nebulized into the chamber and airborne dust materials 1) Phosphate Buffer Solution (PBS) as a control, 2) desert sand from Mongolia and 3) sludge dust from the coastal area of Japan. The result indicated that the co-existence of DH5α with desert dust from Mongolia significantly decreased the viability and with the sludge dust from Japan significantly increased the viability of the airborne DH5α compare to the control PBS dust (p<0.05). Furthermore, soot as a model air pollutant was generated by a candle and mixed with a model airborne bacteria Mycobacterium smegmatis. The results indicated that the different types of airborne environmental dust influenced the viability of airborne bacteria with the DH5α experiments. Furthermore, the presence of soot indicated a possible protective effect of increasing the survival rate of Mycobacterium smegmatis.
Highlights
Desert regions continue to discharge a large amount of, and they circulate around the globe [1]
E. coli solution was prepared in phosphate buffer solution (PBS), and E. coli was 108 to 109 colony forming unit (CFU) per ml
3.1 Dust property For the experiment with E. coli (DH5α) viability test, the effect of Gobi desert dust and Ishinomaki coastal sludge were different dust types were examined which indicate in Figure 4 a) and b), respectively
Summary
Desert regions continue to discharge a large amount of, and they circulate around the globe [1]. These desert dust bring the important minerals to a distant area and supply essential elements as nutrients for various trophic levels. The dust from the Saharan desert was suspected to act as the carrier for biological components such as fungus across the Atlantic Sea to the Caribbean sea to cause coral leaf damage [2]. The aerosols with biological components as bioaerosols can be transported a long distance which may reach downwind and/or surrounding regions to cause possible health effects to human and livestock. We try to understand how the co-existence of different dust or pollutants may act the survival of bioaerosols in laboratory studies
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