Atmospheric chemical processes of microcystin-LR at the interface of sea spray aerosol

Abstract

Microcystins are the most toxic toxins released by cyanobacteria and they have adverse effects on aquatic ecosystems and even human health. Although the removal and detoxification of microcystins in various water bodies have been extensively studied, the interaction mechanism and reaction process of microcystins once they enter the atmosphere are largely unknown, especially at the organic-enriched sea spray aerosol (SSA) interface. Herein, using the surface technique of Langmuir trough coupled in-situ infrared reflection-absorption spectra, we studied the interfacial behavior of microcystin-LR (MC-LR) in artificial seawater containing humic acid and typical surfactants in the presence or absence of UV-irradiation. Zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and anionic stearic acid (SA) were chosen as typical film-forming species, results obtained from the surface pressure-area isotherms showed that MC-LR caused greater expansion of the DSPC monolayer. The comparable results of MC-LR in DSPC/SA-containing systems indicated that the interaction ability was closely related to the monolayer molecular structure and was regulated by electrostatic interaction. Furthermore, the presence of humic acid (HA) could enhance the interaction between microcystin and monolayer molecules. UV-irradiation experiments showed that the photosensitized reaction greatly promoted the removal of microcystin embedded in the SSA surface compared with the direct photolysis effect in the absence of HA. These findings highlight that the toxic effects of microcystins after entering the atmosphere may be weakened by photochemical reactions.