At-line coupling of capillary electrophoresis and surface-enhanced resonance Raman spectroscopy

Abstract

The practicability of an at-line capillary electrophoresis–surface-enhanced resonance Raman spectroscopy configuration was explored. To achieve electrical contact, a metallized outlet capillary was used. The CE effluent was deposited on a moving thin layer chromatography plate. Next, the deposited analytes – three acidic dyes (Food Yellow 3, Acid Orange 7 and Food Red 1) – were treated with silver sol and poly(L-lysine) or nitric acid, followed by irradiation with 514.5 nm light from an argon ion laser to record the SERRS signal. Analyte confirmation could be achieved down to a few pmoles deposited on the TLC plate.