Abstract
BackgroundAtKinesin-13A is an internal-motor kinesin from Arabidopsis (Arabidopsis thaliana). Previous immunofluorescent results showed that AtKinesin-13A localized to Golgi stacks in plant cells. However, its precise localization and biological function in Golgi apparatus is unclear.ResultsIn this paper, immunofluorescent labeling and confocal microscopic observation revealed that AtKinesin-13A was co-localized with Golgi stacks in Arabidopsis root tip cells. Immuno-electron microscopic observations indicated that AtKinesin-13A is primarily localized on Golgi-associated vesicles in Arabidopsis root-cap cells. By T-DNA insertion, the inactivation of the AtKinesin-13A gene (NM-112536) resulted in a sharp decrease of size and number of Golgi vesicles in root-cap peripheral cells. At the same time, these cells were vacuolated in comparison to the corresponding cells of the wild type.ConclusionThese results suggest that AtKinesin-13A decorates Golgi-associated vesicles and may be involved in regulating the formation of Golgi vesicles in the root-cap peripheral cells in Arabidopsis.
Highlights
AtKinesin-13A is an internal-motor kinesin from Arabidopsis (Arabidopsis thaliana)
Root tip cells were used to verify the relationship between AtKinesin-13A localization and individual Golgi stacks marked by Nacetylglucosaminyl transferase I (Nag)-GFP
Confocal microscopic observation revealed that AtKinesin-13A was co-localized with Nag-GFP in Arabidopsis root tip cells (Fig. 1), suggesting that AtKinesin-13A is localized to the Golgi stacks in these cells
Summary
AtKinesin-13A is an internal-motor kinesin from Arabidopsis (Arabidopsis thaliana). Previous immunofluorescent results showed that AtKinesin-13A localized to Golgi stacks in plant cells. Kinesins are a large super-family of microtubule motor proteins that can use the energy of ATP hydrolysis to produce force and move along microtubules [1,2] Based on their motor domain location within the primary sequence of the proteins, different kinesins may have their motor domains affixed at C-terminal, N-terminal or internal positions [3]. The completed Arabidopsis genome contains at least 61 genes encoding polypeptides with the kinesin catalytic core Among these kinesins, AtKinesin-13A and AtKinesin-13B are two internal-motor kinesins [5,6]. Lu et al reported that AtKinesin-13A was co-localized with Golgi stacks in various Arabidopsis cells, indicating that AtKinesin-13A is a special plant internal-motor (page number not for citation purposes)
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