Abstract
The objective of this work was to evaluate ovicidal and larvicidal effects of Eucalyptus globulus essential oil (EGEO) on Haemonchus contortus. The chemical composition determination of EGEO was through gas chromatography and mass spectrometry. Egg hatch test (EHT) was performed in concentrations 21.75; 17.4; 8.7; 5.43 e 2.71 mg x mL(-1). In larval development test (LDT) were used the concentrations 43.5; 21.75; 10.87; 5.43 e 2.71 mg x mL(-1). Each trial was conducted by negative control with Tween 80 (3%) and positive control, 0.02 mg x mL(-1) of thiabendazole in EHT and 0.008 mg x mL(-1) of ivermectin in LDT. The maximum effectiveness of EGEO on eggs was 99.3% in concentration of 21.75 mg x mL(-1) and on larvae was 98.7% in concentration 43.5 mg x mL(-1). The concentration of EGEO that inhibits 50% of the eggs and larvae was 8.3 and 6.92 mg x mL(-1), respectively. The oil chemical analysis identified as main component the monoterpen 1,8-cineol. EGEO presented ovicidal and larvicidal activities in vitro, revealing a good potential for use in the control of sheep and goat gastrointestinal nematodes.
Highlights
As endoparasitoses gastrintestinais constituem-se no principal entrave da ovinocultura, em todo o mundo, especialmente, nas regiões tropicais, onde os prejuízos econômicos são mais acentuados (VIEIRA, 2008)
The chemical composition determination of Eucalyptus globulus essential oil (EGEO) was through gas chromatography and mass spectrometry
Each trial was conducted by negative control with Tween 80
Summary
Foi utilizada uma preparação comercial do óleo essencial de E. globulus (OEEG), adquirido de Dierberguer Óleos Essenciais Ltda (Barra Bonita - São Paulo, lote 0611218). Dois ovinos sem raça definida foram mantidos em gaiolas metabólicas e tratados inicialmente com três anti-helmínticos de diferentes princípios ativos, em dias alternados, para eliminar todos os nematóides gastrintestinais. Foram coletadas 10 g de fezes diretamente da ampola retal de ovino portador de infecção monoespecífica por H. contortus. Para a obtenção das larvas de primeiro estádio (L1), uma alíquota da suspensão de ovos obtida segundo Hubert e Kerboeuf (1992) foi incubada por 24 horas em estufa a 37 °C. Uma alíquota de 1 mL, contendo aproximadamente 250 L1 de H. contortus, foi incubada durante 6 dias com 2 g de fezes provenientes de um animal livre de nematóides gastrintestinais, juntamente com 1 mL do óleo essencial de E. globulus nas concentrações 2,71; 5,43; 10,87; 21,75 e 43,5 mg.mL–1. Foram realizadas três repetições com cinco réplicas para cada tratamento
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