Abstract
Introduction- Urinary tract infections refers to both microbial colonization of urine and tissue invasion of any structure of urinary tract. ASYMPTOMATIC BACTERIURIA (ASB) is a condition in which urine culture reveals significant growth of pathogens that is greater than 1,00,000 bacteria/ml of urine but not showing symptoms of urinary tract infection. Asymptomatic bacteriuria may be of significance or to doubtful significance or of no significance depending on number of bacteria present in urine. ASB is more common in women. Material and Method- The present study was conducted on 200 pregnant women without any symptoms of urinary tract infection attending Obs & Gynae department of RIMS, Ranchi, Jharkhand. It was conducted between August 2013 to December 2014. Urine specimen should be cultured for isolation of the common agents of UTI. The urine should be mixed thoroughly before plating. The plates are inoculated using a calibrated loop designed to deliver a known volume either 0.01 or 0.001 ml. Choice of media to inoculate depends on patient served and microbiologist preference, usually MacConkey Agar and 5% Blood Agar were used. In recent years a selective media CLED (Cysteine lactose electrolyte deficient) agar is used. This media is selective for urinary pathogens cultured plates are incubated at 37⁰C for 24 hrs. Colony count is done>100000/ml of CFU was taken as significant bacteriuria. Kirby Baeur disc diffusion is used for sensitivity test. Result – Asymtomatic bacteriuria is seen in 7.5% cases. It is more common in age group 20- 30 yrs age group and in second trimester of pregnancy. Most common species isolated was E.coli followed by Klebsiella species.Discussion- The percentage of ASB in the present study (7.5%) correlates with the studies of Lavanya SV et al 2002 (8.4%) CA Turpin et al 2007 (7.3%) & R. Sujata et al 2012 (7.3%).In this study, culture positivity with significant bacteriuria was highest in age group 20-30 yrs (73.34%). In 2011 study done by Dr. Rajshekhar maximum incidence was seen in 20-30 yrs age (57.57%).
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