Asymmetry robust centroid localization in confocal microscopy.

Abstract

We present a centroid algorithm with asymmetry-robust error compensation for the peak position localization of asymmetrical axial response signals in confocal microscopy. Compared with the state-of-the-art algorithms, which are usually developed for symmetrical signals, our asymmetry robust centroid algorithm is found to have much smaller localization bias and higher precision for an asymmetrical confocal signal in numerical simulations and experiments.