Abstract
Production of intergeneric somatic hybrids between two sexually incompatible leguminous species, alfalfa (Medicago sativa L.) and birdsfoot trefoil(Lotus corniculatus L.) was carried out. Donor protoplasts of alfalfa were given lethal doses of X-irradiation, and recipient protoplasts of birdsfoot trefoil were inactivated with iodoacetamide. Donor and recipient protoplasts were fused with PEG. Fusion products initiated cell division and colony formation in the culture medium and resulted in callus formation. By using isozyme analysis, it was confirmed that 17 out of 155 calli were somatic cell hybrids. Sorne of the hybrid calli regenerated shoots. By using isozyme analysis it was clarified that the chromosomes or chromosome segments were randomly eliminated from both parents at an early stage of hybrid callus culture. However, after a long period of subculture the isozyme pattern of the recipient type was seen in all of the regenerated shoots which were analyzed. This indicates that the genomes in the calli rapidly converged on the recipient type as subcultures progressed. Southern blot analyses of the chloroplast genomes indicated that either genome of both parents sorted out in all calli investigated.
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