Asymmetric polymerase chain reaction and loop-mediated isothermal amplification (AP-LAMP) for ultrasensitive detection of microRNAs

Abstract

In this manuscript, we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction (A-PCR) and loop-mediated isothermal amplification (LAMP) technology. Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions. We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection. The established method furnished a fast, specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.