Abstract
In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si3N4 Asymmetric Mach–Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab′). We measured a best volumetric sensitivity of 104 rad/RIU, leading to a Limit of Detection below 5 × 10−7 RIU. On sensors functionalized with Fab′, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.
Highlights
In recent years, integrated optical sensors have shown very promising results for label-free detection [1]
We are here interested in a biosensor for a fast and comprehensive detection of Aflatoxin M1 (AFM1) in milk
The surfaces were incubated with an horseradish peroxidase (HRP)-conjugated Aflatoxin M1 (AFM1-HRP) stock solution diluted 80 times in 50 mM
Summary
In recent years, integrated optical sensors have shown very promising results for label-free detection [1]. Even if Surface Plasmon Resonance (SPR) is the most commonly used technique for label-free analysis [2], devices like ring resonators [3,4] and Mach–Zehnder interferometers [5] are showing high sensitivities and miniaturization abilities, which allow realizing a complete lab-on-chip device [6]. The level of AFM1 in milk is analyzed using screening tests like Enzyme-Linked ImmunoSorbent Assay (ELISA). High-Performance Liquid Chromatography is needed when samples show suspect concentration of AFM1. The combination of these analyses is expensive, time-consuming and bulky
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