Astragalus membranaceus Polysaccharide-Enhanced Lymphocytes Proliferation of Yellow Drum Nibea albiflora In Vitro

Abstract

AbstractTo quickly evaluate the immunostimulatory effect of Astragalus membranaceus polysaccharide (AMP) on yellow drum Nibea albiflora, the head kidney lymphocytes proliferation was tested with/without concanavalin A (Con A) or lipopolysaccharide (LPS) in vitro. The lymphocytes were incubated within 0, 25, 50, 100, 200, 400, and 800 µg/mL AMP for 48 h, with/without Con A (2.3 µg/mL) or LPS (18.2 µg/mL). Results showed that there was a dose-dependent relationship between lymphocytes proliferation and the AMP concentration (P < 0.05). The AMP-enhanced lymphocytes proliferation at 50–200 µg/mL significantly (P < 0.05), with peak value at 100 µg/mL. Low dose (0–25 µg/mL) and high dose (400–800 µg/mL) of AMP did not stimulate lymphocytes proliferation significantly (P < 0.05). Synergetic effects were observed between AMP and Con A/LPS on lymphocytes proliferation. Data in this chapter implied that the AMP might contribute to both cellular and humoral immunity of N. albiflora in a dose-dependent manner.Keywords Astragalus membranaceus PolysaccharideLymphocytes proliferation Nibea albiflora