Abstract
Synechococcus sp. PCC7002 can produce theastaxanthin precursor β-carotene but not astaxanthin.Using a synthetic ketolase-and a synthetic hydroxylase-gene we modifiedSynechococcuscarotene metabolism to produce astaxanthin. The total cellular content of astaxanthin was further increased by over-expressing the intrinsic phytoene synthase. Besides a beneficial step in astaxanthin production our results indicate for some modifications in the suggestedprocess of carotenoid metabolism. In addition, we were expressing an E. coli ABC-transporter to export astaxanthinand decrease intracellular product accumulation. By this, the synthesis was increased further and significant amounts of astaxanthin can be recovered from culture medium without cell destruction.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.