Abstract
Glycerol is considered an important renewable feedstock as well as an undesirable side-product of biodiesel production. The aim of this study was to determine whether supplementing a culture medium with a combination of three different carbon sources (olive oil, glucose and glycerol) would optimize lipase production by the yeast Yarrowia lipolytica. The optimization experiments were conducted with a statistical approach using the mixture design. Analysis of the response surface revealed that it would be possible to compose a medium in which both an an extracellular lipase activity of 0.1 U/mL and up to 37.5 g/L of pure glycerol could be obtained. An YPO-Gl30 medium consisting of 30 g/L glycerol and 19.2 mL/L olive oil was selected for further investigation. Although a high biomass yield was found in all cultures, the glycerol content of the YPO-Gl30 medium slightly influenced yeast growth, but it did not prolong the duration of the lag phase. The hydrolytic activity of the extracellular lipases produced in YPO-Gl30 medium was satisfactory.
Highlights
Microbiological research places great importance on the use of microorganisms for environmental protection
The contents of three different carbon sources were the components of the mixture, and the remaining ingredients were used in constant amounts
The glycerol concentration in the medium was based on the results of preliminary studies (Fabiszewska et al 2014) carried out in our laboratory and was not in accordance with that reported by other authors
Summary
Microbiological research places great importance on the use of microorganisms for environmental protection. Such applications often involve the use of specific microorganisms to colonize contaminated environments and attempts to bioconvert waste residues into valuable products. The ability of various bacteria, yeast and fungi to grow on specific carbon and energy sources has led to their use in fermentation systems that utilize waste products. Attempts have been made to use microbial fermentation systems to convert glycerol into 1,3-propanediol, as well as to produce mannitol and erythritol and synthesize invertase. Among the frequently discussed microorganisms with a great potential for applications in future biotechnologies is the yeast species Yarrowia lipolytica (Rywińska et al 2013)
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