Assortative Mating in a Polymorphic Salamander

Abstract

Reproductive isolation due to divergent selection is thought to be one of the mechanisms that promote speciation in sympatry. A key element of reproductive isolation is assortative mating. We examined a polymorphic population of Eastern Red-backed Salamanders (Plethodon cinereus) for evidence of reproductive isolation through assortative mating. Our study population was made up of two common color morphs, striped and unstriped. In the field, we turned over natural cover objects to find male–female pairs of P. cinereus during peak mating season. We recorded sex, color morphology, and snout–vent length of 112 pairs of salamanders. Estimates of sexual isolation indicated weak assortative pairing in the field with more same-color pairs than expected by chance. Striped females paired with striped males were significantly larger in size than those paired with unstriped males. Intermorph pairs were observed and such pairings, if successful, would interfere with the potential for divergence. Laboratory experiment...