Abstract
BackgroundThis study aimed to investigate the differences in oxidative stress (OS) levels represented by 8-iso-prostaglandin F2α (8-iso-PGF2α) and analyze its correlation with the intra-abdominal fat (IAF) area and the glycolipid index.MethodsWe recruited a total of 160 eligible subjects. According to the blood glucose levels and the T2DM duration, subjects were divided into three groups: Type 2 Diabetes (T2DM) group, Prediabetic group, and Normal glucose-tolerance (NC) group, containing 66, 41, 53 patients, respectively. T2DM groups were additionally divided into a new-onset T2DM group including 29 patients and a non-new-onset T2DM group including 37 patients. General clinical data and biochemical indicators were collected. Intra-abdominal fat (IAF) was measured by MRI. 8-iso-PGF2α was measured by ELISA.ResultsCompared with the NC group, levels of systolic blood pressure (SBP), waist-to-hip ratio (WHR), FBG, 2 h postprandial glycemia(2hPG), 2 h insulin (2 h INS), IAF area, HOMA-IR, and 8-iso-PGF2α increased, and high-density lipoprotein cholesterol (HDL-C) decreased in T2DM groups and Prediabetic group (P < 0.05). The 2 h INS level was the highest in the Prediabetic group; 2hPG, and IAF area were the highest in the new-onset T2DM group; WHR, FBG, HOMA-IR and 8-iso-PGF2α were the highest in the non-new-onset T2DM group. Multiple stepwise regression analysis identified IAF area and FBG as the strongest and independent determinant of 8-iso-PGF2α (P < 0.01).ConclusionsIn various glycometabolism populations, 8-iso-PGF2α is significantly correlated with FBG and IAF, this suggests that high blood glucose and abdominal obesity can increase the damage related to the OS in vivo.
Highlights
This study aimed to investigate the differences in oxidative stress (OS) levels represented by 8-isoprostaglandin F2α (8-iso-PGF2α) and analyze its correlation with the intra-abdominal fat (IAF) area and the glycolipid index
Comparison of clinical data and biochemical indicators among different groups There was no difference in age, Body mass index (BMI), DBP, Waist circumference (WC), Hip circumference (HC), TG, TC, LDL-c and Fasting insulin (FINS) among groups (P > 0.05); while systolic blood pressure (SBP), waist-to-hip ratio (WHR), Fasting blood glucose (FBG), 2hPG, 2 h 2 h insulin (INS), IAF area, HOMA-Insulin resistance (IR), and 8-iso-PGF2α were all increased, and high-density lipoprotein cholesterol (HDL-C) decreased in Prediabetic group, New-onset Type 2 diabetes mellitus (T2DM), and non-new onset T2DM groups compared to the Normal glucose-tolerance (NC) group (P < 0.05)
Correlation between 8-iso-PGF2α and different indexes in various impaired glucose-regulation populations Pearson correlation analysis (Table 2) showed that 8-isoPGF2α level in blood was positively correlated with BMI, WC, HC, WHR, FBG, 2hPG, FINS, IAF area, Homeostasis model assessment-insulin resistance (HOMA-IR) (P < 0.05), and negatively correlated with HDL-C (r = − 0.205, P = 0.012)
Summary
This study aimed to investigate the differences in oxidative stress (OS) levels represented by 8-isoprostaglandin F2α (8-iso-PGF2α) and analyze its correlation with the intra-abdominal fat (IAF) area and the glycolipid index. The number of people suffering from diabetes has increased approximately four times from 1980 to 2014, affecting 5% of the world’s population [1]. Chronic inflammation and oxidative stress (OS) have been suggested as important factors in the occurrence and development of Type-2 Diabetes (T2DM) [2, 3]. Oxidative stress increases the mitochondrial electron transport chain (ETC) activity and reactive oxygen species (ROS) production [5, 6]. Previous studies have demonstrated that 8-iso-PGF2α can be used as a relevant factor for predicting the capacity of blood glucose control and internal oxidation in patients with T2DM, as well as a reliable laboratory index for predicting prediabetes (impaired glucose tolerance) [12,13,14]. 8-iso-PGF2α is a valuable biomarker for the evaluation of the level of peroxidation in various glycometabolism populations
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