Associations of gene‑wide SNPs in SNAI1 and TWIST1 with breast cancer and ovarian cancer susceptibility among Chinese Han women

Abstract

Extensive evidence suggests that the genetic etiologies of breast cancer(BC) and ovarian cancer(OC) show a certain degree of similarity. This study aimed to find out whether the single nucleotide polymorphisms(SNPs) of genes SNAI1 and TWIST1 may affect BC and OC susceptibility. A total of 7tagging‑SNPs(tSNPs) were directly genotyped in 1,161BC cases, 286OC cases and 1,273cancer‑free controls among Chinese Han women. Twenty‑eight variants in these 2genes were genotyped by 'in silico' genotype imputation. Logistic regression(LR) revealed that tSNPs SNAI1 rs6125849, TWIST1 rs4721746 and TWIST1 rs4721745 were protective genetic variants for BC/OC. Allelic association tests of gene‑wide SNPs demonstrated that the minor alleles of SNAI1 rs6125849, TWIST1 rs4721745 and TWIST1 rs11973396 were strongly associated with BC/OC susceptibility. Multivariate LR presented that SNAI1 rs6125849, TWIST1 rs4721745, rs4721746 and rs11973396 affected BC/OC susceptibility independently, and women harboring all four protective genoytpes had the lowest risk. Multifactor dimensionality reduction analysis further showed that SNAI1 rs6125849 and TWIST1 rs4721745 had the strongest synergistic interaction. Functional annotation predicted that the minor alleles of SNAI1 rs6125849 and TWIST1 rs4721745 altered their binding affinities with transcription factors E2F6 and TCF11‑MafG respectively. These results indicate that genetic variants in SNAI1 and TWIST1, most probably SNAI1 rs6125849 and TWIST1 rs4721745, may modulate BC and OC susceptibility.