Abstract
Abstract. research because of the large economic impact these discoveries could have on the swine industry. The steroid 21-hydroxylase (CYP21) gene is located on chromosome 7 in the middle of the swine leukocyte antigen class (SLA) is regarded as a "candidate – gene" reproduction traits. Associations between polymorphism of the steroid 21-hydroxylase gene (CYP21) and litter size of Polish Large White × Polish Landrace sows were analysed. The 21-hydroxylase genotypes of all 286 animals were determined using a PCR-RFLP procedure. The frequencies of genotypes and alleles of CYP21/NciI and CYP21/HaeIII were follows: 0.077 – AA, 0.308 – AB, 0.615 – BB and 0.231 for CYP21/NciIA, 0.769 for CYP21/NciIB; 0.010 – AA, 0.420 – AB, 0.570 – BB and 0.221 for CYP21/HaeIIIA and 0.779 for CYP21/HaeIIIB. The CYP21/NciI genotype was significantly associated with the total number of piglets born, born alive and alive at weaning in the 5th – 10th parities. The sows with BB genotype had significantly (P≤0.01) higher level of traits than the animals with AB genotype. Analysis of reproductive traits in dependence on CYP21/NciI genotypes showed the statistically significant differences (P≤0.05) in number of piglets died before the day of weaned in 2-4 parities. The lowest value of this trait was found for the sows with the AB genotype (2.05%), while the highest – for the pigs with BB genotype (3.54%). Associations between CYP21/HaeIII and reproduction traits were not observed.
Highlights
Last years the identification of genes or markers associated with reproductive traits in swine is an important area of research (DROEGEMUELLER et al, 1999; KMIEĆ et al, 2001; SCHLINGMANN et al, 2002; KMIEĆ et al, 2003; MAĆKOWSKI et al, 2004; SCHWARZ et al, 2005) because the large economic impact these discoveries could have on the swine industry
Materials and Methods We examined the association between the CYP21 polymorphism (NciI and HaeIII) and sow reproduction traits in population F1 Polish Landrace × Polish Large White
Amplified 509-bp fragment of DNA was digested with 5 U of HaeIII (GG↓CC) and separated on a 3% agarose gel (Gibco BRL) into 438 bp and 71 bp fragments observed for the AA genotype, and 350 bp, 88 bp and 71bp for the BB genotype (Fig. 2)
Summary
Last years the identification of genes or markers associated with reproductive traits in swine is an important area of research (DROEGEMUELLER et al, 1999; KMIEĆ et al, 2001; SCHLINGMANN et al, 2002; KMIEĆ et al, 2003; MAĆKOWSKI et al, 2004; SCHWARZ et al, 2005) because the large economic impact these discoveries could have on the swine industry. One of the most important enzymatic complexes that take part in the synthesis of adrenal steroids is 21-hydroxylase (CYP21). It takes part in the synthesis of mineralocorticoids and glucocorticoids. The gene encoding 21-hydroxylase (CYP21) is located in the middle of the swine leucocyte antigen class III (SLA) on the short arm of chromosome 7 between the regions SLA class I and SLA class II (GEFFROTIN et al, 1991) which named as “gene – candidate” of swine reproduction traits (ROTHSCHILD and SOLLER, 1997). The aim of this study was to estimate the allelic frequencies at the porcine CYP2/NciI and CYP21/HaeIII loci and to investigate the relationship of those polymorphisms and reproduction traits of Polish Large White × Polish Landrace sows
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