Associations between Interleukin-32 Gene Polymorphisms rs12934561 and rs28372698 and Susceptibilities to Bladder Cancer and the Prognosis in Chinese Han Population.

Jie Yang,Yunjin Bai,Yin Tang,Jia Wang,Zhongyu Jian,Pengfei Shen,Hubertus Himmerich

doi:10.1155/2020/8860445

Jie Yang, Yunjin Bai + Show 5 more

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https://doi.org/10.1155/2020/8860445

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Abstract

The proinflammatory chemokine interleukin-32 is related to various diseases, including cancer. However, it has never been associated with bladder cancer (BC). To detect whether there is a relationship between the IL-32 gene polymorphisms (rs12934561 C/T and rs28372698 T/A) and BC, the study enrolled 170 non-muscle-invasive bladder cancer (NMIBC) patients, 151 muscle-invasive bladder cancer (MIBC) patients, and 437 healthy controls. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for the IL-32 single-nucleotide polymorphism (SNP) genotyping. Statistical analysis was performed using SNPstats online analysis software and SPSS software. Our data revealed that the CC homozygous genotype of rs12934561 in BC patients was significantly higher than that in controls (P = 0.03, OR = 1.47, 95%CI = 1.04‐2.08), and the percentage of TC genotype carriers was relatively less than that of controls (P = 0.001, OR = 0.61, 95%CI = 0.45‐0.82). Furthermore, the TT homozygous genotype of rs28372698 was associated with a significantly lower overall survival rate in MIBC patients (P = 0.028, OR = 2.77, 95%CI = 1.11‐6.90). The IL-32 gene polymorphism rs12934561 might be associated with increased BC risk, and the rs28372698 might participate in the prognosis of BC patients. Therefore, they could be potential forecasting factors for the prognosis of MIBC patients.

Highlights

Bladder cancer (BC) is the tenth most common cancer according to the International Agency for Research on Cancer (IARC), with 549,393 new cases worldwide in 2018 [1]
For rs12934561, the homozygous genotype (CC) in the recessive genetic model was significantly higher in BC patients than that in controls (24.6% vs. 18.1%, P = 0:03, odds ratio (OR) = 1:47, 95%CI = 1:04‐2:08), indicating an increased risk for BC susceptibility
Compared with the TT/CC genotypes, the thyroid cancer (TC) genotype was associated with a lower risk for BC in the overdominant model (P = 0:001, OR = 0:61, 95%CI = 0:45‐0:82)

Summary

Introduction

Bladder cancer (BC) is the tenth most common cancer according to the International Agency for Research on Cancer (IARC), with 549,393 new cases worldwide in 2018 [1]. In 2018, 82,270 new cases and 38,208 deaths were recorded in China, which revealed an estimated increase of 30,000 cases and 20,000 deaths compared with the data of 2012 [1]. According to these reports, only about 20% of BC patients have muscleinvasive bladder cancer (MIBC), which is responsible for most of the cancer-specific deaths. The first-degree relatives of BC patients have a twofold higher risk of developing BC, showing that genetic factors play a crucial role in the initiation and progression of this disease

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