Association Study ofApolipoprotein L-ILys166Glu and Ile244Met Gene Variants with Obesity in Chinese Subjects

Abstract

ApoL-I is a newly found component of high-density lipoprotein, and has a potential role in lipid metabolism. This study was mainly to examine the possible association of the ApoL-I gene polymorphism with obesity and plasma lipid levels in the Chinese population. Three hundred nineteen Han Chinese (109 obese and 210 nonobese control subjects) in Chengdu area were studied using polymerase chain reaction-restriction fragment length polymorphism analysis. The Lys and Glu allele frequencies of the apoL-I gene at the Lys166Glu site in obese and nonobese control groups were 0.830, 0.170 and 0.814, 0.186, respectively. The Ile and Met allele frequencies of the gene at Ile244Met site in the obese and the control groups were 0.844, 0.156 and 0.831, 0.169, respectively. The 166Glu and 244Met allele frequencies of the two polymorphisms in obese subjects were not different from those in the controls, respectively (p>0.05). In the nonobese group, subjects with genotype Lys/Lys at the Lys166Glu site or Ile/Ile at the Ile244Met site had a higher serum mean concentration of triglyceride (TG) as compared with those of corresponding Glu or Met allele carriers (p<0.05). In addition, serum apoC-III and apoE levels were affected by genotypes of Lys166Glu and Ile224Met polymorphisms, respectively, in the control group (all p<0.05). The current study, for the first time, provides evidence that Lys166Glu and Ile244Met polymorphisms in apoL-I gene are associated with TG, apoC-III, and apoE levels in nonobese Chinese. However, these polymorphisms are not associated with obesity in the population.