Association study of IL-10 gene promoter polymorphisms and efficacy of risperidone treatment of schizophrenic patients with first episode

Abstract

Objective To investigate association of IL-10 gene promoter polymorphisms with first episode schizophrenic and efficacy of risperidone. Methods 513 schizophrenic patients with first episode(patient group) and 627 health controls(control group)participated in this study. Genotyping for IL-10 gene label single nucleotide polymorphism(SNP)sites-592A/C (rs1800872), -1082A/G(rs1800896), -819T/C(rs1800871) were performed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP)genotyping technology.513 patients were administered orally risperidone for 8 weeks.Clinical efficacy were evaluated with PANSS. Results The frequences of rs1800896 genotypes (332: 166: 15, 453: 161: 13; χ2=7.55, df=2, P=0.02) and alleles(830: 196, 1067: 187; χ2 =7.09, df=1, P=0.008, OR=1.35, 95%CI=1.08~1.68) showed significant differences between patient group and control group, however only allele differences showed statistical significance after Bonferroni correction(P=0.024). The frequencies of rs1800872and rs1800871 genotypes and alleles had no significant differences between patient group and control group(P>0.05). There were no significant differences of haplotype frequences between patient group and control group(P>0.05). There were no significant differences of genotypes (134: 209: 38, 61: 59: 12; χ2=5.16, df=2, P=0.08; 244: 129: 8, 88: 37: 7; χ2=4.57, df=2, P=0.10; 188: 158: 35, 54: 64: 14; χ2 =2.80, df=2, P=0.25)and alleles (477: 285, 181: 83; χ2=3.03, df=1, P=0.08; 617: 145, 213: 51; χ2 =0.01, df=1, P=0.92; 534: 228, 172: 92; χ2=2.22, df=1, P=0.14) frequences of rs1800872, rs1800896, rs1800871 between effective group(381cases) and invalid group(132 cases) of Risperidone efficacy(P>0.05), while ATC haplotype frequences had significant difference between two groups(χ2=7.501, P=0.006, OR(95%CI)=2.420(1.262-4.640)). Conclusion IL-10 gene rs1800896 polymorphism may be associated with susceptibility to schizophrenia.The clinical curative effect of risperidone in the treatment of schizophrenia is associated with ATC haplotype, ATC haplotype may be good predictors to the overall curative effect of risperidone. Key words: First episode schizophrenia; Gene polymorphisms; Interleukin 10; Risperidone tablets