Abstract
MicroRNAs (miRNAs), important posttranscriptional regulators of gene expression, play a crucial role in plant growth and development. A single miRNA can regulate numerous target genes, making the determination of its function and interaction with targets challenging. We identified PtomiR403b target to PtoGT31B-1, which encodes a galactosyltransferase responsible for the biosynthesis of cell wall polysaccharides. We performed an association study and epistasis and Mendelian randomization (MR) analyses to explore how the genetic interaction between PtoMIR403b and its target PtoGT31B-1 underlies wood formation. Single nucleotide polymorphism (SNP)-based association studies identified 25 significant associations (P < 0.01, Q < 0.05), and PtoMIR403b and PtoGT31B-1 were associated with five traits, suggesting a role for PtomiR403b and PtoGT31B-1 in wood formation. Epistasis analysis identified 93 significant pairwise epistatic associations with 10 wood formation traits, and 37.89% of the SNP-SNP pairs indicated interactions between PtoMIR403b and PtoGT31B-1. We performed an MR analysis to demonstrate the causality of the relationships between SNPs in PtoMIR403b and wood property traits and that PtoMIR403b modulates wood formation by regulating expression of PtoGT31B-1. Therefore, our findings will facilitate dissection of the functions and interactions with miRNA-targets.
Highlights
Trees are an abundant renewable source of pulp and are important in the emerging bioenergy industry (Jansson and Douglas, 2007)
Genomic resequencing of 435 P. tomentosa accessions identified 54 and 165 common Single nucleotide polymorphism (SNP) in PtoMIR403b and PtoGT31B-1, respectively (Supplementary Table 2)
Integration of association studies and Mendelian randomization test, we investigated the allelic interactions between PtoMIR403b and PtoGT31B-1 underlying wood formation
Summary
Trees are an abundant renewable source of pulp and are important in the emerging bioenergy industry (Jansson and Douglas, 2007). Galactosyltransferases are encoded by a small gene family−glycosyltransferase 31 (GT31), include GALT and GALECTIN domains, and mediate the biosynthesis of cell wall polysaccharides, such as xyloglucan (XyG; the dominant component of hemicellulose) and rhamnogalacturonan I (RG-I; a pectin component) (Hennet, 2002; Jensen et al, 2012; Showalter and Basu, 2016; Matsumoto et al, 2019). There are few functional analyses of galactosyltransferase genes in tree species, much of the research being conducted in Arabidopsis thaliana (Fagundes Lopes et al, 2010). Overexpression of EgMUR3 (the MUR3 ortholog in Eucalyptus grandis) in A. thaliana resulted in similar phenotypes, implying a role for galactosyltransferase genes in wood formation (Fagundes Lopes et al, 2010)
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