Association Study and Fine-Mapping MajorHistocompatibility Complex Analysisof Pemphigus Vulgaris in aHanChinese Population.

Abstract

To identify possible additional genetic susceptibility loci for pemphigus vulgaris (PV), we performed a genome-wide association study of 240 PV patients and 1,031 control individuals, and we selected the top single nucleotide polymorphisms for replication in independent samples, with 252 patient samples and 1,852 control samples. We identified rs11218708 (P= 3.1× 10-8, odds ratio= 1.54) at chromosome locus 11q24.1 as significantly associated with PV. A fine-mapping analysis of PV risk in the major histocompatibility complex region showed three independent variants predisposed to PV using stepwise analysis: HLA-DRB1*14:04 (P= 2.47× 10-38, odds ratio= 6.28), rs7454108 at the TAP2 gene (P= 2.78× 10-12, odds ratio= 3.25), and rs1051336 at the HLA-DRA gene (P= 3.06× 10-6, odds ratio= 0.33). A systematic evaluation using gene- and pathway-based analyses showed a high tendency for PV susceptibility genes to be associated with autoimmunity. Our study highlights the involvement of immune-mediated processes in the pathophysiology of PV and illustrates the value of imputation to identify variants in the major histocompatibility complex region.