Abstract
Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens. Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens. Conclusion: These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA.
Highlights
fatty acid (FA) composition analysis: Total lipids in each sample were extracted from breast muscle using chloroform-methanol (2:1) according to a previously reported procedure[10]
Significant differential expression of stearoyl-CoA desaturase (SCD) was found in a previous study[5] and its physiological roles and chromosomal position, which incorporates several QTL related to FA production in chickens, were of particular interest
C18:2, C20:4 and C22:6 polyunsaturated FAs (PUFAs) have been shown to have a positive correlation with the flavor of meat[2], these PUFAs are high in Lantang pig muscles and considered flavor precursors in meat, indicating these pigs have better pork flavor intensity[20]
Summary
FA composition analysis: Total lipids in each sample were extracted from breast muscle using chloroform-methanol (2:1) according to a previously reported procedure[10]. FA methyl esters were prepared from the extracted lipids with BF3-methanol (Sigma-Aldrich, St. Louis, MO, USA). Separated on a HP-6890N gas chromatograph (Hewlett-Packard, Palo Alto, CA, USA) as described previously[11]. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p
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