Abstract
An association of genes XRCC1 and HMMR with breast cancer (BC) has never been tested in the Kyrgyz ethnic group. This was a case-control study of 201 women of the Kyrgyz ethnic group with a morphologically verified breast cancer (N=99) and 102 controls age-matched with BC cases. The mean age of the patients was 53 years (24–74, SE mean = 0.967, STD=9.81). The extraction of DNA was carried out from venous blood. The genotyping was conducted by using the method of polymerase chain reaction and restriction fragment length polymorphism. When comparing the results of genotyping, the histological structure of the tumor and the «menopause» factor, Fisher»s exact test was used. Calculation of the odds ratio was carried out by cross tabulation method. A statistically significant link between the XRCC1 Arg194Trp polymorphism and the menopausal status was observed (p=0,018). The Arg / Arg genotype of XRCC1 Arg194Trp polymorphism occurred in 52 % of cases in women at menopausal age, whereas in women before the onset of menopause, the genotype Arg / Arg occurred in 78.8 % of BC cases (p=0.009). The CT genotype of HMMR V353A polymorphism was identified as “protective” factor – OR=0.481, 95 % CI [0.27‑0.85]. There was no statistically significant association between the results of genotyping and histological structure of the tumor, as well as the age of verification of the diagnosis of BC.
Highlights
An association of genes XRCC1 and HMMR with breast cancer (BC) has never been tested in the Kyrgyz ethnic group. This was a case-control study of 201 women of the Kyrgyz ethnic group with a morphologically verified breast cancer (N=99) and 102 controls age-matched with BC cases
The genotyping was conducted by using the method of polymerase chain reaction and restriction fragment length polymorphism
The Arg / Arg genotype of XRCC1 Arg194Trp polymorphism occurred in 52 % of cases in women at menopausal age, whereas in women before the onset of menopause, the genotype Arg / Arg occurred in 78.8 % of BC cases (p=0.009)
Summary
В исследовании приняли участие 99 женщин кыргы‐ зской этнической группы с морфологически верифици‐ рованным диагнозом рак молочной железы (РМЖ), по‐ лучавшие стационарное лечение в Национальном центре онкологии и Диагностическом центре «КафМедЦентр» В данной работе мы попытались установить нали‐ чие связи между возрастом верификации диагноза РМЖ и генотипом. 1. Впрочем в связи с тем, что генотип Trp / Trp в нашем ис‐ следовании встречается относительно редко (менее 5 %), для проведения дальнейшего статистического анализа па‐ циенты с генотипами Arg / Trp и Trp / Trp были объединены в одну группу Однако статистически значимой связи между возрас‐ том верификации диагноза и генотипами генов XRCC1 (р=0,396), HMMR (р=0,351) выявлено не было. Далее мы попытались установить наличие досто‐ верных различий между распределением генотипов генов XRCC1, HMMR у женщин с наступившей менопа‐ узой и у женщин без менопаузы. Нами была выявлена статистически значимая связь между распределением генотипов полиморфного локуса Arg194Trp гена XRCC1 и фактором «менопау‐. Распределение генотипов XRCC1 Arg194Trp, HMMR V353A в зависимости от возраста больных
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