Abstract
Objective:Vitamin D receptor (VDR) gene polymorphism have a role in diabetes mellitus pathogenesis. Present study was conducted to determine VDR gene variants among Saudi gestational diabetics (GDM) in Madina, KSA.Methods:This cross sectional study was conducted on 112 GDM patients and 218 normal healthy control. Age, body mass index and blood pressure levels were recorded. Serum triglycerides (mg/dl), total cholesterol, HDL-cholesterol, LDL-cholesterol, fasting blood glucose FBG and post-prandial blood glucose PPBG were estimated. Extracted DNA template was amplified by PCR reaction and genotyped for single nucleotide polymorphism of BsmI and FokI by restriction fragment length polymorphism-PCR (RFLP-PCR) analysis.Results:FBG and PPBG levels in GDM patients were significantly elevated by +48.6% and +50%, respectively (P=0.005). Serum triglycerides, total cholesterol and LDL-cholesterol (mg/dl) levels in GDM patients were elevated significantly by +40.5% (P=0.005), +16% (P=0.01) and +30.8% (P=0.005), respectively. Serum HDL-cholesterol (mg/dl) showed significant decline by -10.5%. FokI VDR genotypes showed association with PPBG (P=0.05) among GDM patients. The Ff, FF and ff genotype percentage among GDM patients was 48.2%, 30.4% and 21.4%, respectively. FokI (F and f) and BsmI (B and b) alleles frequency showed no significant difference between GDM patients and control. Percentage BsmI and FokI total homozygous and heterozygous variants among GDM was 45.5% and 81.4%, respectively.Conclusion:VDR BsmI and FokI polymorphic marker not associated with Saudi GDM.
Highlights
Vitamin D receptor (VDR) gene variants may contribute to development of diabetes mellitus through calcium metabolism alteration and modulation of insulin secretion.[4,5,6]
Polymorphism located at 3end of VDR gene
Total number of 112 GDM patients and 218 healthy pregnant women were recruited in our study
Summary
Vitamin D deficiency is associated with diabetes mellitus.[1,2,3] Vitamin D receptor (VDR) gene variants may contribute to development of diabetes mellitus through calcium metabolism alteration and modulation of insulin secretion.[4,5,6]. VDR gene having 6 genes polymorphic forms. Among them; FokI restriction fragment length polymorphism (RFLP) in exon 2 and Bsm[1] RFLPs which located between exons 8 and 9. Polymorphism located at 3end of VDR gene. BsmI (A to G), have unknown functional effects.[7] Positive association between BsmI (genotype bb) polymorphisms with reduced insulin secretory capacity was reported.[8] Diabetics with BB genotype of BsmI allele in VDR gene presented higher levels of C-peptide suggesting association between VDR polymorphism and diabetes.[9]
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