ASSOCIATION OF VARIABLE NUMBER TANDEM REPEAT POLYMORPHISM (VNTR 86 BP) AT RECEPTOR ANTAGONIST INTERLEUKIN-1 (IL-1RA) WITH CLINICAL VARIABLES IN RHEUMATOID ARTHRITIS

Abstract

Event Abstract Back to Event ASSOCIATION OF VARIABLE NUMBER TANDEM REPEAT POLYMORPHISM (VNTR 86 BP) AT RECEPTOR ANTAGONIST INTERLEUKIN-1 (IL-1RA) WITH CLINICAL VARIABLES IN RHEUMATOID ARTHRITIS Sergio J. Ramírez-Pérez1, Samuel García-Arellano1, Ulises De La Cruz-Mosso1, Sergio Cerpa-Cruz2, Yeminia Valle1, Jorge R. Padilla-Gutiérrez1, Edith Oregon-Romero1 and José F. Muñoz-Valle1* 1 Universidad de Guadalajara, Mexico 2 O.P.D. Hospital Civil de Guadalajara “Fray Antonio Alcalde”, Servicio de Reumatología, Mexico BACKGROUND: rheumatoid arthritis (RA) is a chronic systemic-inflammatory disease of unknown etiology characterized by autoantibodies production and cytokines desregulation. There are many factors such as environmental, hormonal, immunological and genetics associated with its susceptibility. Preliminary studies have associated the 86 bp VNTR polymorphism in intron 2 of the IL-1RA gene (rs2234663) to development and susceptibility of rheumatoid arthritis. Based on this knowledge, the aim of this study was to associate the 86 bp VNTR with clinical variables in RA patients from Western Mexico. PATIENTS AND METHODS: we performed an observational case-control study, 183 patients with RA classified according to the ACR/EULAR 2010 criteria and 180 Control Subjects (CS) were analyzed. The identification of IL-1RA VNTR polymorphism was performed by Polymerase Chain Reaction (PCR) technique and visualizated in polyacrylamide gel 6% stained with AgNO3 0.2%. RESULTS: not significant differences in genotype frequencies between cases and controls (p>0.05) were observed. Regarding IL-1RA VNTR alleles, the 2 repeats A2 allele (IL-1RN*2) was associated with protection for RA (OR=0.64, CI 0.44-0.93; p=0.01) in comparisson with A1, A3, and A4 alleles. The genotype frequencies were found in Hardy-Weinberg equilibrium (p=0.35). Subjects carrying of the A1/A2 heterozygous genotype showed a higher disease activity assessed by DAS28 index (5.59; p=0.02), whereas the subjects with genotype A1/A4 showed a significant increase in C-Reactive Protein (CRP) in comparison with other genotypes (31.27 mg/dL vs ; p<0.001). CONCLUSION: the allele A2 is a protector allele for RA in patients from Western Mexico. Moreover, the genotype A1/A2 was associated with increased disease activity, whereas the genotype A1/A4 was associated with a significant increase in CRP. Keywords: Association, polymorphism, IL-1ra, Rheumatoid arthritis, VNTR Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Immunogenetics Citation: Ramírez-Pérez SJ, García-Arellano S, De La Cruz-Mosso U, Cerpa-Cruz S, Valle Y, Padilla-Gutiérrez JR, Oregon-Romero E and Muñoz-Valle JF (2015). ASSOCIATION OF VARIABLE NUMBER TANDEM REPEAT POLYMORPHISM (VNTR 86 BP) AT RECEPTOR ANTAGONIST INTERLEUKIN-1 (IL-1RA) WITH CLINICAL VARIABLES IN RHEUMATOID ARTHRITIS. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00112 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 May 2015; Published Online: 14 Sep 2015. * Correspondence: PhD. José F Muñoz-Valle, Universidad de Guadalajara, Guadalajara, Jalisco, 44340, Mexico, biologiamolecular@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sergio J Ramírez-Pérez Samuel García-Arellano Ulises De La Cruz-Mosso Sergio Cerpa-Cruz Yeminia Valle Jorge R Padilla-Gutiérrez Edith Oregon-Romero José F Muñoz-Valle Google Sergio J Ramírez-Pérez Samuel García-Arellano Ulises De La Cruz-Mosso Sergio Cerpa-Cruz Yeminia Valle Jorge R Padilla-Gutiérrez Edith Oregon-Romero José F Muñoz-Valle Google Scholar Sergio J Ramírez-Pérez Samuel García-Arellano Ulises De La Cruz-Mosso Sergio Cerpa-Cruz Yeminia Valle Jorge R Padilla-Gutiérrez Edith Oregon-Romero José F Muñoz-Valle PubMed Sergio J Ramírez-Pérez Samuel García-Arellano Ulises De La Cruz-Mosso Sergio Cerpa-Cruz Yeminia Valle Jorge R Padilla-Gutiérrez Edith Oregon-Romero José F Muñoz-Valle Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.